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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2017, Article ID 9073172, 8 pages
https://doi.org/10.1155/2017/9073172
Research Article

Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China

1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, China
2College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

Correspondence should be addressed to Jin-sheng Xiu; moc.361@gnehsnijuix and Lun-jiang Zhou; moc.361@gnaijnul

Xue-min Wu and Qiu-yong Chen contributed equally to this work.

Received 8 June 2017; Revised 18 September 2017; Accepted 26 September 2017; Published 31 December 2017

Academic Editor: Jialiang Yang

Copyright © 2017 Xue-min Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the pathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the whole genome of a wild-type pseudorabies virus strain named “FJ-2012.” We then conducted a few downstream bioinformatics analyses including phylogenetic analysis and pathogenic analysis and used the virus to infect 6 pseudorabies virus-free piglets. FJ-2012-infected piglets developed symptoms like high body temperature and central nervous system disorders and had high mortality rate. In addition, we identified typical micropathological changes such as multiple gross lesions in infected piglets through pathological analysis and conclude that the FJ-2012 genome is significantly different from known pseudorabies viruses, in which insertions, deletions, and substitutions are observed in multiple immune and virulence genes. In summary, this study shed lights on the molecular basis of the prevalence and pathology of the pseudorabies virus strain FJ-2012. The genome of FJ-2012 could be used as a reference to study the evolution of pseudorabies viruses, which is critical to the vaccine development of new emerging pseudorabies viruses.