The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance

Table 3

Sequence variation and function of ampGs in clinical P. aeruginosa strains.

	Nucleotide		Amino acid		MIC
	Position	Variation	Position	Variation	(μg/ml)
PA01ΔampG-pUCP24-ampG1	618	C-A	206	/	512/512
PA01ΔampG-pUCP24-ampG2	715	C-T	239	/	512/1024
	1080	G-C	360	/
	1317	C-T	439	/
PA01ΔampG-pUCP24-ampG3	715	C-T	239	/	512/1024
	1080	G-C	360	/
	1317	C-T	439	/
PA01ΔampG-pUCP24-ampG4	715	C-T	239	/	512/1024
	1080	G-C	360	/
	1317	C-T	439	/
PA01ΔampG-pUCP24-ampG7	618	C-A	206	/	512/512
	916	G-C	306	D-H
PA01ΔampG-pUCP24-ampG8	27	C-A	9	/	512/1024
PA01ΔampG-pUCP24-ampG9	27	C-A	9	/	512/512
PA01ΔampG-pUCP24-ampG10	1329	G-A	443	/	512/1024
PA01ΔampG-pUCP24-ampG11	666	C-T	222	/	256/512
	819	G-A	273	/
	942	G-A	314	/
	1062	A-G	354	/
	1077	C-A	539	/
	1113	C-T	371	/
PA01ΔampG-pUCP24-ampG12	618	C-A	206	/	512/1024
PA01ΔampG-pUCP24-ampG13	1423	G-A	475	D-N	512/1024
PA01ΔampG-pUCP24-ampG14	1423	G-A	475	D-N	512/1024
PA01ΔampG-pUCP24-ampG15	336	C-T	112	/	512/4096
PA01ΔampG-pUCP24-ampG16	1747	G-A	583	/	512/1024
PA01ΔampG-pUCP24-ampG17	153	T-C	51	/	512/≥8192
PA01ΔampG-pUCP24-ampG20	213	G-A	71	/	256/4096
PA01ΔampG-pUCP24-ampG24	450	C-T	150	/	1024/4096
PA01ΔampG-pUCP24-ampG25	336	C-T	112	/	1024/≥8192
	1027	C-T	343	/
PA01ΔampG-pUCP24-ampG26	1182	G-A	394	/	512/4096
PA01ΔampG-pUCP24-ampG28	657	C-T	219	/	512/≥8192
	729	C-T	243	/
	837	C-T	279	/
	990	C-T	330	/
	1062	A-G	354	/
	1239	T-C	443	/
	1316	T-C	439	I-T(11)
PA01ΔampG-pUCP24-ampG29	1143	C-T	381	/	512/≥8192
PA01ΔampG-pUCP24-ampG30	27	C-A	9	/	512/≥8192
	1329	G-A	443	/
PA01ΔampG-pUCP24-ampG31	27	C-T	9	/	256/4096
	558	G-A	186	/
	1002	C-T	334	/
	1062	A-G	354	/
	1113	C-T	371	/
PA01ΔampG-pUCP24-ampG35	617	C-T	206	T-I (6)	512/≥8192
	708	T-A	236	/
	1193	G-A	398	G-E (9)
	1364	T-C	455	L-P
PA01ΔampG-pUCP24-ampGPA	/	/	/	/	512
PA01ΔampG	/	/	/	/	32
PA01	/	/	/	/	512
ATCC 25922	/	/	/	/	4

D: aspartic acid; H: histidine; N: asparagine; I: isoleucine; T: threonine; G: glycine; E: glutamate; L: leucine; P: proline; transmembrane region; MIC values of the cloned ORF/wild strain.