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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2019, Article ID 6456321, 6 pages
https://doi.org/10.1155/2019/6456321
Research Article

Antibiotic Resistance Profiles of Haemophilus influenzae Isolates from Children in 2016: A Multicenter Study in China

1Division of Infectious Diseases, The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2Department of Clinical Laboratory, Children’s Hospital of Fudan University, Shanghai 201102, China
3Division of Infectious Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
4Division of Infectious Diseases, Children’s Hospital of Shanghai Jiaotong University, Shanghai 200240, China
5Department of Clinical Laboratory, Children’s Hospital of Shanghai Jiaotong University, Shanghai 200240, China
6Department of Clinical Laboratory, Qilu Children’s Hospital of Shandong University, Jinan 250022, China
7Division of Infectious Diseases, Qilu Children’s Hospital of Shandong University, Jinan 250022, China
8Division of Infectious Diseases, Shanghai Children’s Medical Center, Shanghai 200127, China
9Department of Clinical Laboratory, Shanghai Children’s Medical Center, Shanghai 200127, China
10Department of Clinical Laboratory, Xi’an Children’s Hospital, Xi’an 710043, China
11Division of Infectious Diseases, Xi’an Children’s Hospital, Xi’an 710043, China
12Department of Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China

Correspondence should be addressed to Chun-Zhen Hua; nc.ude.ujz@nehznuhcauh

Received 16 August 2018; Revised 11 February 2019; Accepted 27 February 2019; Published 14 August 2019

Academic Editor: Louis DeTolla

Copyright © 2019 Hong-Jiao Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objective. Haemophilus influenzae (HI) is a common cause of community-acquired pneumonia in children. In many countries, HI strains are increasingly resistant to ampicillin and other commonly prescribed antibiotics, posing a challenge for effective clinical treatment. This study was undertaken to determine the antibiotic resistance profiles of HI isolates from Chinese children and to provide guidelines for clinical treatment. Methods. Our Infectious Disease Surveillance of Pediatrics (ISPED) collaboration group includes six children’s hospitals in different regions of China. The same protocols and guidelines were used by all collaborators for the culture and identification of HI. The Kirby–Bauer method was used to test antibiotic susceptibility, and a cefinase disc was used to detect β-lactamase activity. Results. We isolated 2073 HI strains in 2016: 83.9% from the respiratory tract, 11.1% from vaginal secretions, and 0.5% from blood. Patients with respiratory isolates were significantly younger than nonrespiratory patients (). Of all 2073 strains, 50.3% were positive for β-lactamase and 58.1% were resistant to ampicillin; 9.3% were β-lactamase-negative and ampicillin-resistant. The resistance rates of the HI isolates to trimethoprim-sulfamethoxazole, azithromycin, cefuroxime, ampicillin-sulbactam, cefotaxime, and meropenem were 71.1%, 32.0%, 31.2%, 17.6%, 5.9%, and 0.2%, respectively. Conclusions. More than half of the HI strains isolated from Chinese children were resistant to ampicillin, primarily due to the production of β-lactamase. Cefotaxime and other third-generation cephalosporins could be the first choice for the treatment of ampicillin-resistant HI infections.