Table 1: LD50 of neurovirulence and neuroinvasiveness of chimeric virus and parental viruses.

Dilution of virusJEV SA14-14-2JEV SA14JEV SA14/SA14-14-2
(No. of dead mice/no. of total mice)
i.c.s.c.i.c.s.c.i.c.s.c.

1000/60/6NANA0/60/6
1010/60/6NANA0/60/6
1020/60/66/66/60/60/6
1030/60/66/66/60/60/6
1040/60/66/66/60/60/6
1050/60/66/66/60/60/6
106NANA6/60/6NANA
107NANA1/60/6NANA
Titer of virus (log10 PFU)6.606.606.756.756.546.54
LD50 (PFU)>1.2 ∗ 105>4.0 ∗ 1050.052.1>1.0 ∗ 105>3.5 ∗ 105

NA: not available (represents that the experiments were not carried out).