(a)
(b)
Figure 4: Histopathologic and immunohistochemical findings in the liver of a neotropical nonhuman primate, Alouatta caraya species (monkey #5), that died of YF in Jaboticabal, São Paulo, Brazil, 2016. Asterisks () indicate the portal vein. (a) Moderate (diffuse) lytic necrosis with some Councilman-Rocha Lima (apoptotic) bodies (arrows), mild macrovacuolar steatosis (arrow head). Original magnification ×100, H&E staining. (b) Positive granular and massive intracytoplasmic immunolabeling for the yellow fever virus antigen, with panlobular distribution. Original magnification ×100; anti-YF virus (NDTV/IAL), Warp red™, counter staining with Harrys hematoxylin.