Research Article
Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria
Table 2
Primer sequence/PCR conditions for the MBL, AmpC, KPC, and NDM resistance genotyping [
41–
42].
| | Genes | Primer sequences (5′-3′) | Annealing temperature (°C) | No. of cycles (2–4) | Amplicon size (bp) |
| | VIM | F: GAT GGT GTT TGG TCG CAT
R: CGA ATG CGC AGC ACC AGA | 52 | 36 | 390 | | IMP | F: GGA ATA GAG TGG CTT AAT CTC
R: CCA AAC YAC TAS GTT ATC T | 52 | 36 | 180 | | GIM | F: TCG ACA CAC CTT GGT CTG AA
R: AAC TTC CAA CTT TGC CAT GC | 52 | 36 | 477 | | SPM | F: AAA ATC TGG GTA CGC AAA CG
R: ACA TTA TCC GCT GGA ACA GG | 52 | 36 | 271 | | SIM | F: TAC AAG GGA TTC GGC ATC G
R: TAA TGG CCT GTT CCC ATG TG | 52 | 36 | 570 | | MOXM | F: GCT GCT CAA GGA GCA CAG GAT
R: CAC ATT GAC ATA GGT GTG GTG C | 64 | 25 | 520 | | CITM | F: TGG CCA GAA CTG ACA GGC AAA
R: TTT CTC CTG AAC GTG GCT GGC | 64 | 25 | 462 | | DHAM | F : AAC TTT CAC AGG TGT GCT GGG T
R: CCG TAC GCA TAC TGG CTT TGC | 64 | 25 | 405 | | ACCM | F: AAC AGC CTC AGC AGC CGG TTA
R: TTC GCC GCA ATC ATC CCT AGC | 64 | 25 | 346 | | EBCM | F: TCG GTA AAG CCG ATG TTG CGG
R: CTT CCA CTG CGG CTG CCA GTT | 64 | 25 | 302 | | FOXM | F: AAC ATG GGG TAT CAG GGA GAT G
R: CAA AGC GCG TAA CCG GAT TGG | 64 | 25 | 190 | | NDM-1 | F: ACC GCC TGG ACC GAT GAC CA
R: GCC AAA GTT GGG CGC GGT TG | 58 | 35 | 264 | | KPC | F: CATTCAAGGGCTTTCTTGCTGC
R: ACGACGGCATAGTCATTTGC | 55 | 30 | 538 |
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