Distinct Patterns of Host Adherence by Neisseria gonorrhoeae Isolated from Experimental Gonorrhea

Figure 4

The (a) adherence and (b) internalization of reisolates and input strain (MS11) to human Chang conjunctiva cells. The level of adherence to cells was calculated by determining the cell-associated CFUs of the host cell monolayers (coincubation for 1.5 to 6 h after an incubation period of 24 h). For internalization, an approach similar to adherence assays by killing the extracellular bacteria with 100 μg/ml (final concentration) gentamicin as previously described. The number of internalized bacteria was determined by counting CFUs recovered following gentamicin treatment. . . .

(a)

(b)