Research Article
Carbapenemase Production and Detection of Colistin-Resistant Genes in Clinical Isolates of Escherichia Coli from the Ho Teaching Hospital, Ghana
Table 2
Primers for detection of colistin-resistant genes.
| Target gene | Primer | Sequence (5′-3′) | Amplification product (bp) | Annealing temperature (°C) | Reference |
| mcr-1 | mcr-1F | ATCAGCCAAACCTATCCCATCG | 1257 | 55 | [18] | mcr-1R | GCAGACGCACAGCAATGCCTAT |
| mcr1_320 bp | mcr1_320 bp_F | AGTCCGTTTGTTCTTGTGGC | 320 | 58 | [19] | mcr1_320 bp_R | AGATCCTTGGTCTCGGCTTG |
| mcr-2 | mcr-2F | GCGATGGCGGTCTATCCTGTAT | 378 | 55 | [18] | mcr-2R | TGCGATGACATGGGGTGTCAGC |
| mcr-2_700 bp | mcr2_700 bp_F | CAAGTGTGTTGGTCGCAGTT | 715 | 58 | [19] | mcr2_700 bp_R | TCTAGCCCGACAAGCATACC |
| mcr-3 | mcr-3F | TATGGGTTACTATTGCTGG | 814 | 55 | [18] | mcr-3R | CTACCCTGATGCTCATCG |
| mcr3_900 bp | mcr3_900 bp_F | AAATAAAAATTGTTACGCTTATG | 929 | 58 | [19] | mcr3_900 bp_R | AATGGAGATCCCCGTTTTT |
| mcr-4 | mcr-4F | GTCATAGTGGTATAAAAGTACAG | 669 | 55 | [18] | mcr-4R | CCACCGTCTATCAGAGCCAAC |
| mcr_4_1100 bp | mcr_4_1100 bp_F | TCACTTTCATCACTGCGTTG | 1116 | 58 | [19] | mcr_4_1100 bp_R | TTGGTCCATGACTACCAATG |
| mcr-5 | mcr5_F | ATGCGGTTGTCTGCATTTATC | 1644 | 58 | [20] | mcr5_R | TCATTGTGGTTGTCCTTTTCTG |
| mmcr-5 | mcr-5F | GCGGTTGTCTGCATTTATCAC | 1042 | 50 | [18] | mcr-5R | CTTTGAAAACCTGTCTTTGGCA |
| mcr-6 | mcr-6F | GTCCGGTCAATCCCTATCTGT | 556 | 55 | [18] | mcr-6R | ATCACGGGATTGACATAGCTAC |
| mcr-7 | mcr-7F | TGCTCAAGCCCTTCTTTTCGT | 892 | 55 | [18] | mmcr-7R | TTCATCTGCGCCACCTCGT |
| mcr-8 | mcr-8F | AACCGCCAGAGCACAGAATT | 667 | 60 | [18] | mcr-8R | TTCCCCCAGCGATTCTCCAT |
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