CD44v6-Targeted Imaging of Head and Neck Squamous Cell Carcinoma: Antibody-Based Approaches

Figure 4

Phage display selection from a protein library. Protein libraries are displayed on phage particles as fusions to coat proteins. Each phage displays a unique protein and encapsulates the encoding DNA, which links the genotype and phenotype of the displayed protein. Protein variants (e.g., antibodies) that bind an immobilized antigen are isolated through rounds of binding selection and amplification. Nonbinding phages are removed by washing. Retained phages are recovered, amplified by bacterial infection, and cycled through additional rounds of selection. Compared to immunization-based methods, in vitro selection enables full control of library design and selection conditions. Binding clones are identified through sequencing of the encapsulated DNA.