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Contrast Media & Molecular Imaging
Volume 2017, Article ID 3127908, 8 pages
https://doi.org/10.1155/2017/3127908
Research Article

Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques

1CEA, Université Paris Sud 11, INSERM U1184, Immunology of Viral Infections and Autoimmune Diseases Research Center, IDMIT Infrastructure, 92265 Fontenay-aux-Roses, France
2Vaccine Research Institute (VRI), Créteil, France
3CEA, Institute of Biomedical Imaging (I2BM), DSV/I2BM/SHFJ/INSERM U1023, CEA, Orsay, France

Correspondence should be addressed to Catherine Chapon; rf.aec@nopahc.enirehtac

Received 24 February 2017; Revised 28 April 2017; Accepted 8 May 2017; Published 7 June 2017

Academic Editor: Fijs W. B. Van Leeuwen

Copyright © 2017 Biliana Todorova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates. Procedures. Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM). Results. The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy. Quantification of Langerhans cells revealed no changes to cell density with time. Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis. Conclusions. This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.