Multimodal Imaging Nanoparticles Derived from Hyaluronic Acid for Integrated Preoperative and Intraoperative Cancer Imaging

Figure 3

Ratio optimization of SAMINs. To achieve optimal contrast in both MR and fluorescent imaging modalities, the concentration of Cy7.5 must be calibrated to the ideal concentration of gadolinium for MRI contrast. (a) Fluorescence emission spectra of SAMINs with varying ratios of Gd : Cy7.5. The maximum fluorescence intensity as a function of concentration is shown in (b), from which the optimal ratio is derived. MR and NIR fluorescence images of the formulations are presented to illustrate the process of optimizing the ratio of gadolinium to Cy7.5.

(a)

(b)