Breast Tumor Microcalcification Induced by Bone Morphogenetic Protein-2: A New Murine Model for Human Breast Tumor Diagnosis

Figure 2

Microscopic detection of alkaline phosphatase activity and mineralization of C2C12 cells induced by rhBMP-2: microscopic detection of alkaline phosphatase in C2C12 cells (a) in presence and absence of rhBMP-2. The differentiation of C2C12 cells to osteoblast-like cells was achieved in differentiation medium for 21 days. The mineralization of differentiated cells induced by rhBMP-2 was detected using alizarin red (b) and von Kossa staining (c) as compared to controls treated with PBS. Magnification = 200x.