Magnetosomes Extracted from Magnetospirillum gryphiswaldense as Theranostic Agents in an Experimental Model of Glioblastoma

Figure 2

In vitro characterization of MNs. (a) TEM of whole-mount of bacteria (scale bar, 500 nm). (b) MNs after isolation of M. gryphiswaldense (scale bar, 100 nm). (c, d) TEM of the internalization of MNs with U87MG cells: MNs are visible in cytoplasm vacuoles (scale bar, (c) 2000 nm and (d) 1000 nm). (e, f) Cellular uptake: representative histological images of MNs-treated cells after 24 h of incubation with 0.2 and 0.5 mg/ml of MNs (Prussian blue staining; scale bar, 50 µm). (g) MTT assay on U87MG cells after incubation with MNs: MTT assay shows a negligible cytotoxic effect of MNs, percent viability of cells incubated with MNs is expressed relative to control cells. Error bars refer to SEM.