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Contrast Media & Molecular Imaging
Volume 2018, Article ID 3181258, 7 pages
https://doi.org/10.1155/2018/3181258
Research Article

Dynamic Contrast-Enhanced Imaging as a Prognostic Tool in Early Diagnosis of Prostate Cancer: Correlation with PSA and Clinical Stage

1Department of Oncology, Tampere University Hospital, Tampere, Finland
2Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland
3Department of Radiology, Medical Imaging Centre, Tampere University Hospital, Tampere, Finland
4Department of Medical Physics, Medical Imaging Centre, Tampere University Hospital, Tampere, Finland

Correspondence should be addressed to Xingchen Wu; if.atu@uw.nehcgnix

Received 10 April 2018; Accepted 22 July 2018; Published 19 September 2018

Academic Editor: Orazio Schillaci

Copyright © 2018 Xingchen Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Purpose. Although several methods have been developed to predict the outcome of patients with prostate cancer, early diagnosis of individual patient remains challenging. The aim of the present study was to correlate tumor perfusion parameters derived from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and clinical prognostic factors and further to explore the diagnostic value of DCE-MRI parameters in early stage prostate cancer. Patients and Methods. Sixty-two newly diagnosed patients with histologically proven prostate adenocarcinoma were enrolled in our prospective study. Transrectal ultrasound-guided biopsy (12 cores, 6 on each lobe) was performed in each patient. Pathology was reviewed and graded according to the Gleason system. DCE-MRI was performed and analyzed using a two-compartmental model; quantitative parameters including volume transfer constant (Ktrans), reflux constant (Kep), and initial area under curve (iAUC) were calculated from the tumors and correlated with prostate-specific antigen (PSA), Gleason score, and clinical stage. Results. Ktrans (0.11 ± 0.02 min−1 versus 0.16 ± 0.06 min−1; ), Kep (0.38 ± 0.08 min−1 versus 0.60 ± 0.23 min−1; ), and iAUC (14.33 ± 2.66 mmoL/L/min versus 17.40 ± 5.97 mmoL/L/min; ) were all lower in the clinical stage T1c tumors (tumor number, ) than that of tumors in clinical stage T2 (). Serum PSA correlated with both tumor Ktrans (, ) and iAUC (, ). Conclusions. Our study has confirmed that DCE-MRI is a promising biomarker that reflects the microcirculation of prostate cancer. DCE-MRI in combination with clinical prognostic factors may provide an effective new tool for the basis of early diagnosis and treatment decisions.

1. Introduction

Prostate cancer is the second leading cause of cancer-related death and the most frequently diagnosed male malignant disease in the Nordic countries [1]. Early detection of prostate cancer permits appropriate and timely management of the disease, and prognostic biomarkers can help clinicians to make a proper decision for treatment of individual patients and to avoid unnecessary treatments [2]. Although several methods have been developed to predict outcome of patients with prostate cancer, prognosis evaluation of individual patient remains challenging. Recent studies demonstrate that multiparametric magnetic resonance imaging (MP-MRI), consisting of T1-weighted, T2-weighted, diffusion-weighted imaging (DWI), and dynamic contrast-enhanced MRI (DCE-MRI), has emerged as a useful tool not only for localizing prostate cancer foci, but also for assessing tumor aggressiveness [3]. DWI allows to quantify the random motion of water molecules in tissue by means of apparent diffusion coefficient (ADC) measurements and provides information on tissue cellularity, tortuosity of extracellular space, and cell membrane integrity, thereby differentiating noncancerous and cancer lesions [4]. DCE-MRI is a relatively novel imaging modality that allows to measure properties of tissue microvasculature resulting from tumor angiogenesis and improving tumor detection and response assessment [5]. The most commonly used DCE-MRI parameter that reflects vascular permeability is the volume transfer constant (Ktrans) [6]. Ktrans represents the rate at which the contrast agent transfers from the blood to the interstitial space, which indicates the tumor microcirculation and the surface infiltration area. In contrast, the reflux constant (Kep) reflects the rate at which the contrast agent transfers from the extravascular extracellular space back to the blood. The extravascular extracellular leakage volume fraction (Ve = Ktrans/Kep) predominantly reflects the percentage of contrast agent in the extravascular extracellular space [6]. In addition, the semiquantitative parameter initial area under curve (iAUC) is associated with tumor blood influx, perfusion, and interstitial space and represents the general tumor blood flow, overall perfusion, and tumor interstitial space index [6].

The aim of the present study was to correlate tumor perfusion parameters derived from DCE-MRI and clinical prognostic factors and further to explore if we can separate very early tumors from relatively advanced ones with DCE-MRI-derived parameters for decision making in early stage prostate cancer.

2. Materials and Methods

2.1. Patients

Seventy-one consecutive patients with histologically proven prostate adenocarcinoma were enrolled in our prospective clinical trials to develop hypofractionated image-guided and intensity-modulated radical radiotherapy. The study identifier at www.ClinicalTrials.gov is NCT02319239. The inclusion and exclusion criteria have been described in details in our previous publication [7]. Briefly, newly diagnosed adult patients with one or two of the intermediate-risk features (Gleason score 7, staging T2b-T2c, PSA 10–20 ng/mL) according to the National Comprehensive Cancer Network (NCCN) criteria [8], and patients were suitable for MRI examination. No patients received neoadjuvant or adjuvant hormonal treatment. The study was approved by the Ethics Committee of Tampere University Hospital (Nr. R14009), and all patients gave written informed consent prior to study entry. Patients underwent physical examination, digital rectal examination, and standard laboratory tests including serum prostate-specific antigen (PSA).

2.2. Histological Analysis

Transrectal ultrasound-guided biopsy (12 cores, 6 on each lobe) was performed in each patient. Six biopsy cores were embedded in one paraffin block. Pathology was reviewed and graded according to the Gleason system. Major criteria include an infiltrative glandular growth pattern and an absence of basal cells and nuclear atypia in the form of nucleomegaly and nucleolomegaly. The diagnosis was based on the microscopic appearance of slides stained using haematoxylin and eosin. In difficult cases, basal cell absence has been confirmed by immunohistochemical stains for basal cell markers.

2.3. Multiparametric MRI Acquisition

Multiparametric MR imaging was acquired using a 3 Tesla MR System (Siemens Trio-Tim, Erlangen, Germany) with a combination of 6-channel body matrix coil and 6 elements of 24-channel spine matrix coil positioned around the pelvis to cover the prostate. Tri-planar T2-weighted turbo spin echo images from below the prostatic apex to above the seminal vesicles were obtained. DWI was acquired with a single-shot echoplanar sequence on the axial plane using three b values (50, 400, and 800 s/mm2) and with the same orientation and location used to acquire axial T2-weighted images. DCE-MRI was performed with axial T1-weighted 3D volumetric interpolated breath-hold examination (VIBE) sequence that covers the entire prostate in consecutive sections. To determine the T1 relaxation time in the tissue before the arrival of contrast agent, the DCE-MRI included two precontrast 3D VIBE imaging sequences that had different flip angles (2° and 13°). These sequences were followed by a DCE series on the axial plane after gadolinium (Gd)-DOTA (0.2 ml/kg Dotarem®) injection, with a temporal resolution of 8 seconds and an acquisition time of 4 minutes 40 seconds. The contrast agent was administered using a power injector (Medrad Spectris Solaris EP, Bayer Medical Care Inc, PA, USA) followed by a 20 ml saline flush injection at a flow rate of 2.5 ml/s. To minimize postbiopsy artifact, MRI was performed 6–10 weeks after the prostate cancer confirmation by biopsy. For imaging parameters, see Table 1.

Table 1: Sequence parameters for 3T multiparametric MRI with the body and spine matrix combination coil system.
2.4. MR Image Analysis

All MR images were reviewed and analyzed on a syngo Multimodality Workplace (Siemens Healthcare). Voxelwise MRI signal enhancement time curves were fitted according to a pharmacokinetic model using Tissue 4D software (Siemens Healthcare). First, a motion correction has been performed, which registered all volumes of the time series to a user-selected reference volume to reduce the effect of patient and physiological motion during the DCE image acquisition. After the registration of the morphological image and the precontrast image, an oval-shaped or irregular-shaped region of interest (ROI) was drawn on the prostate cancer foci. ROIs were drawn in early enhancing region of DCE-MRI and with the DWI b800, ADC map, and T2-weighted image as references. T1 map calculation of precontrast was a prerequisite for pharmacokinetic modeling. T1 fitting was restricted to pixels with values above a noise level value (>20), and the respective values were automatically calculated by the system as a function of the entered contrast agents. For the Tofts modeling [6], Tissue 4D provides arterial input function (AIF) that are modeled using a biexponential function with three different modes (fast, intermediate, and slow). The AIF was chosen according to the fast sampling method to calculate kinetic parameters [9]. Parametric maps were calculated, and Ktrans, Kep, Ve, and iAUC of the selected ROI were automatically estimated by the software.

The ADC value of each identified tumor lesion was measured directly on the parametric ADC maps. The ADC map was reviewed simultaneously with the corresponding high b value DW images, T2-weighted images, and precontrast T1-weighted images. The slice of the ADC map containing the largest tumor extent was selected for analysis, and a ROI was drawn in the center of the tumor excluding the tumor edges. The mean ADC value and the size of the selected tumor area were generated at the workstation and recorded for analysis.

A prostate cancer was defined on each MRI as follows: a hypointense region relative to the adjacent parenchyma on T2-weighted image; a region with a low ADC value relative to the adjacent parenchyma on the ADC map; and a region with early wash-in and wash-out of contrast medium relative to the adjacent parenchyma on DCE-MRI. Precontrast T1-weighted images were used to identify postbiopsy hemorrhage (as an area with high signal intensity) to rule out false-positive findings.

2.5. Statistical Analysis

Statistical analysis was performed with SPSS (version 23.0, SPSS Inc., Chicago, Illinois, USA). A two-sided nonparametric Mann–Whitney U test was used to compare the patients age, PSA, tumor size, ADC, Ktrans, Kep, Ve, or iAUC between the peripheral and transitional zone tumor groups, between Gleason score 3 + 3 and 3 + 4 groups, and between different clinical stages. Spearman’s correlation coefficient was used to evaluate the correlation between tumor size, ADC, Ktrans, Kep, Ve, iAUC, Gleason score, and serum PSA; values less than 0.05 were considered significant.

3. Results

3.1. Patient Characteristics

No suspicious lesion was found on MRI in 7 out of the 71 patients with a biopsy proven prostate cancer; two patients had no DCE images due to allergy to the contrast agent. Sixty-nine lesions were detected in the prostate of the remaining 62 patients (age: mean ± SD: 70 ± 5 years, range from 60 to 79 years). Ten patients had clinical stage T1c and 52 had T2 (16 in T2a, 8 in T2b, and 28 in T2c) tumors according to TNM classification for prostate cancer. The serum PSA value (mean ± SD) was 9.5 ± 3.7 ng/mL, with the range from 3.4 to 19.1 ng/mL.

3.2. Pathological Results

There were 19 patients with a Gleason score 3 + 3, 41 with a Gleason score 3 + 4, and 2 with a Gleason score 4 + 3 tumor.

None of the measured parameters, including patients’ age, serum PSA, and DWI- and DCE-MRI-derived parameters, were different between Gleason score 3 + 3 and 3 + 4 tumor groups.

3.3. Tumor Location

The majority of the tumors were in the peripheral zone (52, 75%), and the other 17 tumors were in the transitional zone.

There was no significant difference of the patients’ age, serum PSA, tumor ADC, Ktrans, or iAUC between the peripheral and transitional zone tumor groups (Table 2).(a)The size of peripheral zone tumors (lesion number, ) was smaller than that of the transitional zone tumors () (0.68 ± 0.41 cm2 versus 0.93 ± 0.59 cm2; ).(b)Kep was higher in the peripheral zone tumors (lesion number, ) than that of the transitional zone tumors () (0.59 ± 0.21 min−1 versus 0.49 ± 0.24 min−1; ).(c)Ve was lower in the peripheral zone tumors (lesion number, ) than that of the transitional zone tumors () (0.27 ± 0.08 versus 0.32 ± 0.07; ).

Table 2: Comparison of the 62 patients with peripheral and transitional zone prostate cancer (46 versus 16): age, tumor size, and DWI- and DCE-derived tumor parameters.
3.4. DCE-MRI-Derived Parameters

Prostate cancer showed earlier and more pronounced enhancement than surrounding normal prostate tissue (example Figure 1). Fifty-nine patients had perfusion MRI findings of at least one focal enhancing tumor in the prostate. In three patients, focal lesions were not obvious on the DCE images; all these 3 patients had clinical stage T2c tumors.

Figure 1: Transverse prostate MR images from a 69-year-old male patient with biopsy proven prostate cancer (Gleason score 3 + 4 and serum PSA 6.6 ng/mL): (a) T2-weighted image showing in the transitional zone a hypointense area without clear border; (b) ADC map: transitional zone hypointense region with a clear border, with ADC value of 0.75 × 10−3 mm2/s; (c) T1-weighted image early enhancement map: the enhanced region of interest 1 (ROI1, red line) corresponds to the tumor, and ROI 2 (green line) was selected from normal prostate tissue as healthy control; (d) Ktrans map: ROI 1 Ktrans 0.120 min−1 and ROI 2 Ktrans 0.048 min−1; (e) Kep map: ROI 1 Kep 0.657 min−1 and ROI 2 Kep 0.327 min−1; (f) iAUC map: ROI 1 iAUC 14.976 mmoL/L/min and ROI 2 iAUC 6.871 mmoL/L/min; (g) enhancement kinetics pattern from the two ROIs: the time-intensity curves were obtained from dynamic contrast-enhanced MRI. ROI1 showing a higher peak enhancement and an early wash-in and wash-out of contrast medium compared with ROI 2.

Ktrans (0.11 ± 0.02 min−1 versus 0.16 ± 0.06 min−1; ), Kep (0.38 ± 0.08 min−1 versus 0.60 ± 0.23 min−1; ), and iAUC (14.33 ± 2.66 mmoL/L/min versus 17.40 ± 5.97 mmoL/L/min; ) were all lower in the clinical stage T1c tumors () than that of the clinical stage T2 tumors () (Figures 2(a)2(c)).

Figure 2: Comparison of tumor Ktrans, Kep, iAUC, and serum PSA level in patients with different clinical stages of prostate cancer. (a) Ktrans (0.11 ± 0.02 min−1 versus 0.16 ± 0.06 min−1; ), (b) Kep (0.38 ± 0.08 min−1 versus 0.60 ± 0.23 min−1; ), and (c) iAUC (14.33 ± 2.66 mmoL/L/min versus 17.40 ± 5.97 mmoL/L/min; ) were all lower in clinical stage T1c tumors than that in clinical stage T2 tumors; (d) there was no significant difference of serum PSA between clinical stage T1c and T2 patients (8.2 ± 4.5 ng/mL versus 9.8 ± 3.6 ng/mL; ).
3.5. Serum PSA Value

There were no significant differences of the serum PSA levels between clinical stage T1c () and T2 patients () (8.2 ± 4.5 ng/mL versus 9.8 ± 3.6 ng/mL; ) (Figure 2(d)).

3.6. The Correlations between PSA and MRI Parameters

Serum PSA correlated with both tumor Ktrans (, ) (Figure 3(a)) and tumor iAUC (, ) (Figure 3(b)).

Figure 3: Correlations between serum PSA and DCE-MRI-derived tumor parameters in the 62 patients with prostate cancer. (a) Serum PSA correlated with tumor Ktrans (, ); (b) Serum PSA correlated with tumor iAUC (, ).

No correlation was found between serum PSA and tumor ADC value.

4. Discussion

A reliable diagnostic test should be able to provide an early prostate cancer diagnosis and minimize the amount of unnecessary biopsies or treatments. From this perspective, morphological MRI is a good candidate for prostate cancer investigation as it provides high-contrast and high-resolution images of the prostate. However, no single MRI sequence is sufficient to characterize prostate cancer. Each of the functional MR components has clinical advantages and limitations. Early promising data suggest that MP-MRI, which is performed concurrently with anatomical and functional techniques, is the most sensitive and specific imaging tool for lesion detection, characterization, and staging of prostate cancer [3]. Our study revealed a correlation between tumor Ktrans and serum PSA in patients with early stage prostate cancer. This finding is consistent with previous publications [10, 11]. In addition, we detected a correlation between tumor iAUC and serum PSA. These may be explained by the altered vascular permeability of tumor microvessels and lymphatic system [12]. Neovascularity has been demonstrated to be a prerequisite for tumor growth and metastasis [13]. Abnormal angiogenesis in the tumor tissue lead to higher microvessel density, which is represented by leakage, twisted morphology, vascular wall expansion, and crosslinking [14]. Many scientists have suggested microvessel density as a prognostic and a predictive factor [15]. However, microvessel density measurement depends on the availability of postoperative tissue or biopsy materials, and it is a static assessment rather than information on vascular function. Therefore, there were controversial results between microvessel density and prostate cancer progression and grade [16]. In contrast, the distribution of Gd-DOTA in DCE-MRI is determined not only by microvessel density but also by vessel permeability and size of the extravascular extracellular space. DCE-MRI not only provides more details in tumor morphology but also allows to assess contrast agent kinetics and thus allows to improve detection and grading of prostate cancer.

DCE-MRI can be used to assess noninvasively the functional aspects of microcirculation of tissues. DCE-MRI relies on the fact that a bolus of contrast agent passing through the capillary bed is transiently confined within the vascular space before passing rapidly into the extravascular extracellular space at a rate determined by the permeability of the microvessels, their surface area, and blood flow [17, 18]. In DCE-MRI, the distribution of the contrast agent is repeatedly measured, allowing the evaluation of the tumor microcirculation in vivo and enabling the malignancy or benignancy of the tumor to be quantitatively distinguished [19]. Neoangiogenesis plays a vital role in the growth, progression, and metastasis process of prostate cancer [20, 21]. Microvessel density in prostatic carcinoma has also been shown to be an independent predictor of the pathological stage [13]. In consistent, we found that the tumor Ktrans, Kep, and iAUC were all lower in smaller tumors (T1c) than in larger local tumors (T2) in biopsy proven prostate cancer. To our knowledge, this is the first report that revealed DCE-MRI-derived parameters can separate very early stage tumors and relatively advanced tumors in clinically localized prostate cancer. Quantification of tumor angiogenesis by DCE-MRI may allow stratification of patients to type of treatment.

Serum PSA is elevated as a result of disruption of the prostatic architecture in the presence of prostate disease and injury, and PSA screening helps to diagnose prostate cancer earlier, at lower clinical stages and with lower Gleason score [22]. However, we did not find significant difference of the serum PSA levels between the tumors in clinical stage T1c and those with relatively extent diseases, for example, clinical stage T2. Serum PSA is not a specific marker for prostate cancer because of variable contribution to PSA from benign tissue and the nonlinear relationship between grade and PSA, which lead to overlap in PSA levels between different clinical stages as shown also in previous studies [23]. As a result, serum PSA level cannot be used alone to accurately predict disease extent for any individual patient. DCE-MRI play a role in conjunction with PSA for localizing suspicious lesions for biopsy, improving specificity, and identifying those tumors that are more likely to cause death if they are left untreated.

The Gleason score reflects the tumor aggressiveness and is an important predictor of outcome in patients with prostate cancer [2]. Correlation between the Gleason score and DCE-MRI-derived parameters may have been expected, because the Gleason scores have been shown to correlate with microvessel density measurements [13]. However, we did not detect any significant difference of the DCE-derived parameters between patients with Gleason score 3 + 3 and 3 + 4. The lack of differences may be explained by the heterogeneity of tumor tissues [24] and the histological sampling errors inherent in needle biopsy. Secondly, our patients were selected with one or two of the intermediate-risk features. Therefore, the differences of their disease extent/magnitude are relatively small compared with previous publications [10, 11].

Our study has a few limitations: firstly, the MRI was performed after biopsy. We were not sure, if the tumor ADC value and DCE parameters had been measured at the biopsy sites. Secondly, we were unable to evaluate the correlation between MRIs and histopathological features accurately because we did not obtain surgical specimens. There have been concerns about the probability of undergrading prostate cancer by biopsy due to tumor heterogeneity. Thirdly, all patients underwent needle biopsies before MRI examinations, implying that hemorrhagic or inflammatory changes caused by this procedure might have affected the MRIs. However, we excluded visible bleeding with the help of precontrast T1-weighted images, and the time interval between biopsy and MRI was long (6–10 weeks) enough for biopsy wound healing.

5. Conclusions

In conclusion, the present study has confirmed that DCE-MRI is a promising biomarker that reflects the microcirculation of prostate cancer. DCE-MRI-derived quantitative parameters in combination with clinical prognostic factors may provide an effective pretreatment diagnosis modality for early prostate cancer, especially for those with negative biopsy.

Data Availability

The data are available from the Medical Imaging Center of Tampere University Hospital.

Conflicts of Interest

The authors have no conflicts of interest to declare.

Acknowledgments

This project was supported by the Competitive State Research Financing of the Expert Responsibility Area of Tampere University Hospital, Seppo Nieminen Fund (Grant no. 150613), and Pirkko Kellokumpu-Lehtinen (Grant nos. 9R019 and 9S021). Xingchen Wu was supported by the Finnish Cultural Foundation, Pirkanmaa Regional Fund, and the Finnish Medical Foundation. The authors would like to thank research coordinator Irja Kolehmainen for her contributions.

References

  1. M. M. Center, A. Jemal, J. Lortet-Tieulent et al., “International variation in prostate cancer incidence and mortality rates,” European Urology, vol. 61, no. 6, pp. 1079–1092, 2012. View at Publisher · View at Google Scholar · View at Scopus
  2. J. Cuzick, M. A. Thorat, G. Andriole et al., “Prevention and early detection of prostate cancer,” The Lancet Oncology, vol. 15, no. 11, pp. e484–e492, 2014. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Dickinson, H. U. Ahmed, C. Allen et al., “Magnetic resonance imaging for the detection, localisation, and characterisation of prostate cancer: recommendations from a European consensus meeting,” European Urology, vol. 59, no. 4, pp. 477–494, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. C. Sato, S. Naganawa, T. Nakamura et al., “Differentiation of noncancerous tissue and cancer lesions by apparent diffusion coefficient values in transition and peripheral zones of the prostate,” Journal of Magnetic Resonance Imaging, vol. 21, no. 3, pp. 258–262, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. N. Hylton, “Dynamic contrast-enhanced magnetic resonance imaging as an imaging biomarker,” Journal of Clinical Oncology, vol. 24, no. 20, pp. 3293–3298, 2006. View at Publisher · View at Google Scholar · View at Scopus
  6. P. S. Tofts, G. Brix, D. L. Buckley et al., “Estimating kinetic parameters from dynamic contrast-enhanced T(1)-weighted MRI of a diffusable tracer: standardized quantities and symbols,” Journal of Magnetic Resonance Imaging, vol. 10, no. 3, pp. 223–232, 1999. View at Publisher · View at Google Scholar
  7. X. Wu, P. Reinikainen, A. Vanhanen et al., “Correlation between apparent diffusion coefficient value on diffusion-weighted MR imaging and Gleason score in prostate cancer,” Diagnostic and Interventional Imaging, vol. 98, no. 1, pp. 63–71, 2017. View at Publisher · View at Google Scholar · View at Scopus
  8. NCCN, National Comprehensive Cancer Network Clinical Practice Guidelines in Oncology: Prostate Cancer, NCCN, Fort Washington, PA, USA, 2014, Version 1.
  9. M. R. Orton, J. A. d’Arcy, S. Walker-Samuel et al., “Computationally efficient vascular input function models for quantitative kinetic modelling using DCE-MRI,” Physics in Medicine and Biology, vol. 53, no. 5, pp. 1225–1239, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. M. P. Chung, D. Margolis, S. Mesko, J. Wang, P. Kupelian, and M. Kamrava, “Correlation of quantitative diffusion-weighted and dynamic contrast-enhanced MRI parameters with prognostic factors in prostate cancer,” Journal of Medical Imaging and Radiation Oncology, vol. 58, no. 5, pp. 588–594, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. E. Cho, D. J. Chung, D. M. Yeo et al., “Optimal cut-off value of perfusion parameters for diagnosing prostate cancer and for assessing aggressiveness associated with Gleason score,” Clinical Imaging, vol. 39, no. 5, pp. 834–840, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Verma, B. Turkbey, N. Muradyan et al., “Overview of dynamic contrast-enhanced MRI in prostate cancer diagnosis and management,” American Journal of Roentgenology, vol. 198, no. 6, pp. 1277–1288, 2012. View at Publisher · View at Google Scholar · View at Scopus
  13. M. K. Brawer, R. E. Deering, M. Brown, S. D. Preston, and S. A. Bigler, “Predictors of pathologic stage in prostatic carcinoma. The role of neovascularity,” Cancer, vol. 73, no. 3, pp. 678–687, 1994. View at Publisher · View at Google Scholar
  14. M. Furuya and Y. Yonemitsu, “Cancer neovascularization and proinflammatory microenvironments,” Current Cancer Drug Targets, vol. 8, no. 4, pp. 253–265, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Muhammadnejad, A. Muhammadnejad, M. Haddadi et al., “Correlation of microvessel density with nuclear pleomorphism, mitotic count and vascular invasion in breast and prostate cancers at preclinical and clinical levels,” Asian Pacific Journal of Cancer Prevention, vol. 14, no. 1, pp. 63–68, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. P. Yuri, A. Z. Hendri, and R. Danarto, “Association between tumor-associated macrophages and microvessel density on prostate cancer progression,” Prostate International, vol. 3, no. 3, pp. 93–98, 2015. View at Publisher · View at Google Scholar · View at Scopus
  17. J. Ren, Y. Huan, H. Wang et al., “Dynamic contrast-enhanced MRI of benign prostatic hyperplasia and prostatic carcinoma: correlation with angiogenesis,” Clinical Radiology, vol. 63, no. 2, pp. 153–159, 2008. View at Publisher · View at Google Scholar · View at Scopus
  18. M. de Rooij, E. H. Hamoen, J. J. Futterer, J. O. Barentsz, and M. M. Rovers, “Accuracy of multiparametric MRI for prostate cancer detection: a meta-analysis,” American Journal of Roentgenology, vol. 202, no. 2, pp. 343–351, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Jackson, J. P. O’Connor, G. J. Parker, and G. C. Jayson, “Imaging tumor vascular heterogeneity and angiogenesis using dynamic contrast-enhanced magnetic resonance imaging,” Clinical Cancer Research, vol. 13, no. 12, pp. 3449–3459, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. G. Russo, M. Mischi, W. Scheepens, J. J. De la Rosette, and H. Wijkstra, “Angiogenesis in prostate cancer: onset, progression and imaging,” BJU International, vol. 110, no. 11c, pp. E794–E808, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. G. Bergers and L. E. Benjamin, “Tumorigenesis and the angiogenic switch,” Nature Reviews Cancer, vol. 3, no. 6, pp. 401–410, 2003. View at Publisher · View at Google Scholar · View at Scopus
  22. H. B. Carter, “Differentiation of lethal and non lethal prostate cancer: PSA and PSA isoforms and kinetics,” Asian Journal of Andrology, vol. 14, no. 3, pp. 355–360, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. S. Pentyala, T. Whyard, S. Pentyala et al., “Prostate cancer markers: an update,” Biomedical Reports, vol. 4, no. 3, pp. 263–268, 2016. View at Publisher · View at Google Scholar · View at Scopus
  24. R. J. Gillies, P. A. Schornack, T. W. Secomb, and N. Raghunand, “Causes and effects of heterogeneous perfusion in tumors,” Neoplasia, vol. 1, no. 3, pp. 197–207, 1999. View at Publisher · View at Google Scholar