Isolated Perfused Rat Livers to Quantify the Pharmacokinetics and Concentrations of Gd-BOPTA

Figure 2

Protocol of the isolated perfused rat livers. (a) A gamma counter detects online the count rates coming from a region of interest (ROI) delineated within a rat liver lobe. The system avoids traumatic collection of small biopsies over time. (b) We standardise the perfusion protocol to compare results in various experimental conditions. Rat livers are perfused successively with Gd-DTPA that distributes into the extracellular space and Gd-BOPTA that enters into hepatocytes after extracellular distribution. Between drug perfusions, Krebs–Henseleit bicarbonate (KHB) solutions are perfused (rinse periods).

(a)

(b)