Isolated Perfused Rat Livers to Quantify the Pharmacokinetics and Concentrations of Gd-BOPTA

Figure 3

Gd-BOPTA distribution in rat livers. (a) The livers are perfused with 500 µM Gd-DTPA (♦, n = 4 ), 500 µM Gd-BOPTA (□, n = 4), 500 µM Gd-BOPTA and 500 µM bromosulfophthalein (BSP, ◯, n = 4). An additional group of rats with acute bile duct ligation is perfused with 500 µM Gd-BOPTA (▲, n = 2). BSP perfusion prevented Gd-BOPTA uptake. (b) Gadolinium concentrations in portal veins (◯), hepatic veins (△), and hepatic tissues (■) during Gd-BOPTA perfusion. Additional livers are perfused with Gd-DTPA (●) and Gd-BOPTA + BSP (□). Three liver biopsies are collected at each time point. (c) Representative MR images of a liver perfused with Gd-DTPA and Gd-BOPTA (upper panels) or DTPA and Gd-BOPTA + BSP (lower panels). Adapted from [20] with permission.