Contrast Media & Molecular Imaging / 2018 / Article / Fig 3

Research Article

NIRF Optical/PET Dual-Modal Imaging of Hepatocellular Carcinoma Using Heptamethine Carbocyanine Dye

Figure 3

Preferential uptake and retention of NIRF dye in orthotopic HCC xenograft tumors in mice. (a) NIRF imaging of Hep3B-3.1 cell-derived tumor xenografts and D68979 PDX tumor xenografts in orthotopic models. HCC Hep3B-3.1-Luc cells and a tumor fragment from a PDX model were implanted into the livers of nude mice. Two weeks later, tumor-emitting signals were captured by NIRF imaging. Representative images are shown. (b) Quantification of NIRF dye uptake in (a) (per cm2). Data are presented as the ratio of dye uptake intensity as normalized to that of blank region (mean ± SD, ). (c) Ex vivo dual BLI/NIRF imaging of select organs, including the heart, liver, spleen, lung, and kidney, as dissected from mice in (a). (d) Quantification of NIRF signal intensity from the tumor-bearing experimental group in (c). Data are presented as the percentage (mean ± SD, ) of signal intensity as normalized to that of liver tumor. Signal intensity in liver tumor is set as 100%. (e) H&E analyses of tumor tissues derived from orthotopic HCC xenograft tumors. Original magnification, ×400; scale bars represent 20 μm.

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