Evans Blue Dye: A Revisit of Its Applications in Biomedicine

Figure 2

Postmortem analysis of necrotic and viable liver from rats with reperfused partial liver infarction that received iodine-123-labeled monoiodohypericin followed by the necrosis-avid dye, Evans blue [33]. At 4, 24, and 48 hours (h) after radioactivity injection, liver necrosis is outlined by the Evans blue as a blue region (A1, B1, and C1), with viable liver without staining (A1′, B1′, and C1′). Autoradiograms of 50 μm thick sections show higher tracer accumulation in the hepatic infarction (A2, B2, and C2) than in viable liver (A2′, B2′, and C2′). The color code bar represents the coding scheme for the radioactivity. On histologic sections, the presence of scattered liver necrosis (A3, B3, and C3) and the location of the normal liver (A3′, B3′, and C3′) are confirmed (reprinted and modified with permission from Miranda Cona M, Koole M, Feng Y, Liu Y, Verbruggen A, Oyen R, Ni Y. International Journal of Oncology 2014, 44: 819–829).