Multimodality Molecular Imaging-Guided Tumor Border Delineation and Photothermal Therapy Analysis Based on Graphene Oxide-Conjugated Gold Nanoparticles Chelated with Gd

Figure 3

(a) Temperature elevation of aqueous solution of GO-AuNS-DOTA-Gd nanoparticles at different concentrations over 10 min under NIR laser irradiation (808 nm, 2 W) recorded every 10 seconds with a digital thermometer; (b) temperature elevation of aqueous solution of GO-AuNS-DOTA-Gd nanoparticles over five LASER ON/OFF cycles of NIR laser irradiation (808 nm, 2 W) (LASER ON time: 10 min; LASER OFF time: 30 min); (c) photothermal destruction of HCC-LM3-fFluc cells with different treatments. C1: no irradiation and no agent; C2: agent of 25 μg/mL only; C3: 5 min irradiation, no agent; C4: 5 min irradiation, agent of 25 μg/mL. White dashes indicate the laser boundary; dead cells are labeled in red, while viable cells are labeled in green; (d) cell viability after treatment with different concentrations of GO-AuNS-DOTA-Gd nanoparticles and different NIR laser irradiation time. and .

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