Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients

Table 1

List of primary antibodies.

Antibody	Characteristics	Dilution	Retrieval
Antivimentin	Mouse monoclonal clone V9; Ventana, Tucson, AZ, USA	Prediluted	EDTA citrate pH 7.8
Anti-BMP-2	Rabbit monoclonal clone N/A; Novus Biologicals, Littleton, CO, USA	1 : 250	Citrate pH 6.0
Anti-PTX-3	Rat monoclonal clone MNB1; AbCam, Cambridge, UK	1 : 100	Citrate pH 6.0
Anti-RUNX2	Mouse monoclonal clone EPR14334; AbCam, Cambridge, UK	1 : 100	Citrate pH 6.0
Anti-RANKL	Rabbit monoclonal clone 12A668; AbCam, Cambridge, UK	1 : 100	EDTA citrate pH 7.8
Anti-VDR	Rabbit polyclonal clone NBP1-19478; Novus Biologicals, Littleton, CO, USA	1 : 100	Citrate pH 6.0