Research Article

Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation

Figure 4

Biodistribution, autoradiography (ARG), and VCAM-1 expression results of the rabbits and colons. (a) The biodistribution of 99mTc-scFv-VCAM-1 in rabbits showed tracer uptake in different tissue and organs after 3 hours of probe administration (mean ± SD, ). (b) The ARG confirmed tracer uptake in the model colon, with weak accumulation in the control and the blocked colon. (c) Semiquantitative analysis of the ARG region of interest showed more radioactivity in the model group than that in the control and the blocked group (mean ± SD, ). (d) Western blotting indicated stronger VCAM-1 expression in the model colon than that in the control colon. (e) Immunohistochemistry study demonstrated more VCAM-1 expression in blood vessels of the model colon (upper) than that of the control colon (lower). The strongest VCAM-1 expression (black arrows) located on vascular endothelial cells in the model colon.