Cellular MRI Reveals Altered Brain Arrest of Genetically Engineered Metastatic Breast Cancer Cells

Figure 1

In vitro characterization of engineered 4T1BR5 cell line: after lentiviral transduction, 4.3% of the total population was GFP positive (purple = GFP negative; green = GFP positive) (sort 1) (a). These cells were grown out and found to be 86.6% GFP positive during the second sort. From this population, we sorted out the brightest 8.2% of cells (purple = cells not collected; green = 8.2% brightest GFP expression that were collected) (sort 2) (b). Brightfield and GFP expression of resultant 4T1BR5-Fluc-GFP cells (scale bar = 100 microns) (c). Luciferase activity of 4T1BR5-Fluc-GFP cells over multiple passages (P13–P25) in culture (d). Cell cycle arrest analysis of 4T1BR5 (e) and 4T1BR5-Fluc-GFP cells (f). Cellular proliferation of naïve 4T1BR5 and 4T1BR5-FLuc-GFP cells (g). Data are presented as mean ± SEM.

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