Cellular MRI Reveals Altered Brain Arrest of Genetically Engineered Metastatic Breast Cancer Cells

Figure 2

Altered initial brain arrest of engineered 4T1BR5 cells as detected with iron oxide cellular MRI: Perl’s Prussian blue staining identifies iron-labeled cells in blue (scale bar = 50 microns) (a). Iron-labeled cells were visualized in brain MR images as discrete signal voids on day 0 ( per group); insets showing example of distinct voids (b). Brain and body BLI signal was also detectable on day 0 in mice that received luciferase-expressing cells () (c). The number of voids (in MR images) on day 0 in mice receiving naïve 4T1BR5 cells or 4T1BR5-Fluc-GFP cells (d). The data are presented as mean ± SEM.

(a)

(b)

(c)

(d)