Multiplexed ¹²⁹Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells

Figure 3

HyperCEST MR Z-spectra and images of GV cells. The strengths of saturation RF pulses employed for the HyperCEST MR Z-spectra and images were 1.5 μT (30 dB) and 5.9 μT (52 dB), respectively. (a) Z-spectrum of GV_AC28 cell and control KPL-4 cell samples with 8.0 × 10⁷ cells/ml (upper) and GV_AC16C20 cell sample with 2.0 × 10⁷ cells/ml (lower). Color triangles indicate the saturation offsets used to calculate %CEST contrast in (b). (b) Saturation time dependency of %CEST contrast for GV_AC28 cells (upper) and GV_AC16C20 cells (lower). (c) HyperCEST MR images of GV_AC28 cell and control KPL-4 cell samples with 8.0 × 10⁷ cells/ml. The dotted yellow line indicates the wall of the 10φ glass tube in which the cell suspension was contained. The color bar indicates %CEST contrast.

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