Research Article

Exosome-Derived Circ_0094343 Promotes Chemosensitivity of Colorectal Cancer Cells by Regulating Glycolysis via the miR-766-5p/TRIM67 Axis

Figure 2

Characterization of exosomes from NCM460 cells. (a) After negative staining of exosomes with uranyl acetate, the morphology of exosomes in the exo-vector group and the exo-circ group was observed by transmission electron microscopy (TEM); (b) nanoparticle tracker to detect the diameter of exosomes in the exo-vector group and the exo-circ group; (c) Western blot to determine the protein expression level of HSP70 and CD63 in exosomes and cell lysates in each group; (d) qRT-PCR for determination of circ_0094343 expression level in exosomes in each group; (e) PKH67 fluorescent labeling was performed to determine the level of exosome uptake by HCT116 cells. .
(a)
(b)
(c)
(d)
(e)