Research Article

Exosome-Derived Circ_0094343 Promotes Chemosensitivity of Colorectal Cancer Cells by Regulating Glycolysis via the miR-766-5p/TRIM67 Axis

Figure 7

Exosome circ_0094343 inhibits the proliferation and glycolysis and improves the chemosensitivity of HCT116 cells through the miR-766-5p/TRIM67 axis. (a) qRT-PCR for detection of the mRNA expression level of TRIM67 in cells of each group; (b), (c) Western blot was used to determine the protein expression level of TRIM67 in cells of each group; (d) MTT for checking the proliferation rate of cells in each group; (e), (f) The clone formation ability of the cells in each group was evaluated using clone formation assay; (f)–(i), kits to detect the glucose consumption level (g), the lactate production level (h), and the level of ECAR (i) of the cells in each group; (j), (l) MTT for determination of the survival rate in each group of cells treated with different concentrations (0, 0.5, 1, 5, 10, 50 μM) of 5-FU (j), L-OHP (k), and Dox (l). vs., NC, and vs., exo-circ, vs., miR-776-5p mimics.