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Computational and Mathematical Methods in Medicine
Volume 2017, Article ID 8967902, 9 pages
Research Article

Box-Counting Method of 2D Neuronal Image: Method Modification and Quantitative Analysis Demonstrated on Images from the Monkey and Human Brain

1Department of Biophysics, School of Medicine, University of Belgrade, Višegradska 26/2, 11000 Belgrade, Serbia
2Department of Anatomy, School of Medicine, University of Novi Sad, Hajduk Veljkova 21, 21000 Novi Sad, Serbia

Correspondence should be addressed to Nebojša Milošević;

Received 24 January 2017; Revised 28 March 2017; Accepted 9 April 2017; Published 8 May 2017

Academic Editor: Dong Song

Copyright © 2017 Nemanja Rajković et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study calls attention to the difference between traditional box-counting method and its modification. The appropriate scaling factor, influence on image size and resolution, and image rotation, as well as different image presentation, are showed on the sample of asymmetrical neurons from the monkey dentate nucleus. The standard BC method and its modification were evaluated on the sample of 2D neuronal images from the human neostriatum. In addition, three box dimensions (which estimate the space-filling property, the shape, complexity, and the irregularity of dendritic tree) were used to evaluate differences in the morphology of type III aspiny neurons between two parts of the neostriatum.