Research Article

Iterative Variable Gene Discovery from Whole Genome Sequencing with a Bootstrapped Multiresolution Algorithm

Figure 3

Comparison of TBLASTN hits compared to the V-gene sequences positively identified by VgeneFinder: (a) log(eValue) scores and (b) scores of TBLASTN for all candidates. The histogram negative candidates (red) are candidate sequences that VgeneFinder has discarded. The V-gene sequence histograms (yellow) are positively identified by VgeneFinder. The plots show that just based on TBLASTN homology, there would be no manner to determine positive and negative sequences; TBLASTN and similar homology methods are not effective for this task nor could they be used to automatically classify the exons into their respective loci.
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