[Retracted] Breast Cancer Cells Metastasize to the Tissue-Engineered Premetastatic Niche by Using an Osteoid-Formed Polycaprolactone/Nanohydroxyapatite Scaffold

Figure 2

A coculture system was established by using varisized cell culture inserts. hMSCs were seeded onto the upper insert (24-well plate culture insert). After 46 days of osteogenic differentiation, osteoid-formed PCL-nHA scaffolds were seeded onto the middle insert (12-well plate culture insert). Breast cancer cells were seeded onto the lower chamber (12-well plate). hMSCs: human mesenchymal cells; PCL: polycaprolactone; nHA: nanohydroxyapatite.