Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers

Table 1

Primer sequences used in MSP.

Gene	Primer
FITH-M	Forward: 5-GGTTTTTACGCGCGTTAGGT-3
	Reverse: 5-GCTCATAAAAAGCAAAATGCTCC-3
FITH-U	Forward: 5-GGTTTTTATGTGTGTTAGGT-3
	Reverse: 5-ACTCATAAAAAACAAAATACTCC-3
P16-M	Forward: 5-TTATTAGAGGGTGGGGCGGATCGC-3
	Reverse: 5-GACCCCGAACCGCGACCGTAA-3
P16-U	Forward: 5-TTATTAGAGGGTGGGGTGCATTGT-3
	Reverse: 5-CAACCCCAAACCACAACCATAA-3
MGMT-M	Forward: 5-TTTCGACGTTCGTAGGTTTTCGC-3
	Reverse: 5-GCACTCTTCCGAAAACGAAACG-3
MGMT-U	Forward: 5-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3
	Reverse: 5-AACTCCACACTCTTCCAAAAACAAAACA-3
RASSF1A-M	Forward: 5-GGGTTTTGCGAGAGCGCG-3
	Reverse: 5-GCTAACAAACGCGAACCG-3
RASSF1A-U	Forward: 5-GGTTTTGTGAGAGTGTGTTTAG-3
	Reverse: 5-CACTAACAAACACAAACCAAAC-3
APC-M	Forward: 5-TATTGCGGAGTGCGGGTC-3
	Reverse: 5-TCGACGAACTCCCGACGA-3
APC-U	Forward: 5-GTGTTTTATTGTGGAGTGTGGGTT-3
	Reverse: 5-CCAATCAACAAACTCCCAACAA-3
DAPK-M	Forward: 5-GGATAGTCGGATCGAGTTAACGTC-3
	Reverse: 5-CCCTCCCAAACGCCGA-3
DAPK-U	Forward: 5-GGAGGATAGTTGGATTGAGTTAATGTT-3
	Reverse: 5-CAAATCCCTCCCAAACACCAA-3