Research Article

[Retracted] Downregulation of miR-146a-5p Promotes Acute Pancreatitis through Activating the TLR9/NLRP3 Signaling Pathway by Targeting TRAF6 In Vitro Rat Model

Figure 1

miR-146a-5p is highly expressed in cerulein-induced pancreatitis. (a–c) After treating AR42J cells with 0, 2.5, 5, and 10 nM cerulein, ELISA detected the levels of inflammatory factors in the culture medium, with 10 μg/mL LPS and 10 mM ATP as positive controls. (d, e) The expression of inflammation-related miRNAs in AR42J cells with 10 nM cerulein was detected by RT-qPCR, with 10 μg/mL LPS and 10 mM ATP as positive controls. (f) RT-qPCR was used for disclosing the expression of miR-146a-5p in cerulein-induced AR42J cells with miR-146a-5p mimics or mimics nc. (g–i) ELISA detected the expression of inflammatory factors in the culture medium of cerulein-induced AR42J cells. (j) CCK8 detected the proliferation of cerulein-induced AR42J cells. (k) Flow cytometry (FCM) assay detected the apoptosis of cerulein-induced AR42J cells. , , and . The experiments were repeated three times, the statistical test was a two-tailed test, and errors bars in the figures represent standard deviation (SD).
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