Research Article

[Retracted] Downregulation of miR-146a-5p Promotes Acute Pancreatitis through Activating the TLR9/NLRP3 Signaling Pathway by Targeting TRAF6 In Vitro Rat Model

Figure 2

miR-146a-5p targets TRAF6. (a) miR-146a-5p and TRAF6-3UTR binding site as indicated by prediction software BiBiServ and RNAfold. (b) Dual-luciferase detection of miR-146a-5p and TRAF6-3UTR binding. (c, d) qPCR and WB detection of the expression of TRAF6 after adding miR-146a-5p mimics. Band analysis software is ImageJ (version 1.8.0_172). (e, f) qPCR and WB inspection of the expression of TRAF6 in AR42J cells treated with 0, 2.5, 5, and 10 nM cerulein. Band analysis software is ImageJ (version 1.8.0_172). , , and . The experiments were repeated three times, the statistical test was a two-tailed test, and errors bars in the figures represent standard deviation (SD).
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