Research Article

[Retracted] Downregulation of miR-146a-5p Promotes Acute Pancreatitis through Activating the TLR9/NLRP3 Signaling Pathway by Targeting TRAF6 In Vitro Rat Model

Figure 3

Knockdown of TRAF6 inhibits the development of pancreatitis. (a) 24 h after transfection of TRAF6-siRNA, qPCR detects the knockdown efficiency of siRNA on TRAF6 in 10 nM cerulein-induced AR42J cells. (b) 48 h after transfection of TRAF6-siRNA, WB detected the knockdown efficiency of siRNA on TRAF6 in 10 nM cerulein-induced AR42J cells. Band analysis software is ImageJ (version 1.8.0_172). (c–e) The levels of inflammatory factors in the culture medium were detected by ELISA in 10 nM cerulein-induced AR42J cells. (f) CCK8 detected the effect of TRAF6 knockdown on cell proliferation in 10 nM cerulein-induced AR42J cells. (g) FCM detected the influence of TRAF6 knockdown on cell death in 10 nM cerulein-induced AR42J cells. , , and . The experiments were repeated three times, the statistical test was a two-tailed test, and errors bars in the figures represent standard deviation (SD).
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