[Retracted] miR-335-5p Inhibits Progression of Uterine Leiomyoma by Targeting ARGLU1

Figure 4

ARGLU1 mediates the inhibitory effects of miR-335-5p on the UL cell lines. (a) The viability of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic or cotransfected with miR-335-5p mimic and pc-ARGLU1 was assessed by CCK-8 assay at indicated times. The proliferation of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic or cotransfected with miR-335-5p mimic and pc-ARGLU1 was assessed by (b) colony formation and (c) EdU assays. (d) The expression levels of proliferation-related proteins, including PCNA and Ki-67, were evaluated by western blot assay. (e) The cell cycle distribution of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic or cotransfected with miR-335-5p mimic and pc-ARGLU1 was assessed by flow cytometry analysis assay. (f) The expression levels of cell cycle-related proteins, including Cyclin A1, Cyclin B1, Cyclin D2, and p27, were evaluated by western blot assay. (g) The cell apoptosis of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic or cotransfected with miR-335-5p mimic and pc-ARGLU1 was assessed by flow cytometry analysis assay. (h) The expression levels of cell apoptosis-related proteins, including Bcl-2, Bax, Cleaved caspase-3, and Cleaved caspase-9, were evaluated by western blot assay. vs. the NC mimic+pc-NC group. ^# and ^##vs. the miR-335-5p mimic+pc-NC group. All data were presented as the . .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)