MMP1 Overexpression Promotes Cancer Progression and Associates with Poor Outcome in Head and Neck Carcinoma

Figure 4

MMP1 expression pattern during HNSCC tumorigenesis in 4NQO-induced animal model. (a) Experimental scheme of 4NQO-induced HNSCC animal model. (b–i) Immunohistochemical staining of MMP1 in samples from diverse stages in 4NQO-induced animal model. Images in the upper panel (b, d, f, h) were representative staining of MMP1 in normal, epithelial with hyperplasia, epithelial with severe dysplasia/carcinoma in situ, and squamous cell carcinoma, respectively. Images in the lower panel (c, e, g, i) were magnified from the black box area in the (b, d, f, h) images in the upper panel, respectively. Scale bar: 100 μm. (j) The mRNA levels of MMP1 during the 4NQO-induced HNSCC were measured by qRT-PCR in mice samples ( =5 samples per group). , , ANOVA analyses.

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