A Framework to Predict the Molecular Classification and Prognosis of Breast Cancer Patients and Characterize the Landscape of Immune Cell Infiltration

Figure 3

Immune-cell infiltration (ICI) score construction. (a) Alluvial diagram showing the distribution of ICI gene clusters in different ICI score groups and survival status of TCGA-BRCA patients. (b) Box plot for the relative expression of immune checkpoint-associated genes in different ICI score groups. Among them, CD274, HAVCR2, CTLA4, LAG3, PDCD1, and IDO1 are inhibitory genes, and CD8A, GZMA, PRF1, CXCL10, CXCL9, TNF, and TBX2 are stimulatory genes. The asterisks represent the statistical P-value (Kruskal-Wallis test, ). GESA enrichment maps for (c) high and (d) low ICI score groups. Allograft rejection, E2F targets, G2M checkpoint, interferon gamma response, and MYC target V2 pathways were enriched in the high ICI score group. Epithelial mesenchymal transition, estrogen response early, protein secretion, TGF-β signaling, and UV response pathways were enriched in the low ICI score group. (e) Survival analysis for high or low ICI score groups (calculated by using PCA) in TCGA-BRCA patients using Kaplan-Meier curves. The log-rank test showed that .

(a)

(b)

(c)

(d)

(e)