Angiotensin II Mediates Cardiomyocyte Hypertrophy in Atrial Cardiomyopathy via Epigenetic Transcriptional Regulation

Figure 3

AngII-induced atrial cardiomyocyte hypertrophy. Atrial cardiomyocytes were stimulated with saline, AngII, AngII + losartan, AngII + the PKD inhibitor CID755673, or AngII + the CaMKII inhibitor KN93 for 48 h and subjected to immunofluorescence staining with antibody against α-actin. (a) Cell surface area of atrial cardiomyocytes treated with different stimuli was examined. The mean surface area of atrial cardiomyocytes after treatment was obtained from 200 atrial cardiomyocytes per sample. (b) Atrial cardiomyocyte extracts from different groups were subjected to western blotting using anti-ANP, BNP, SKA, and β-MHC antibodies. Data are plotted as fold enrichment over equivalent amounts of GAPDH. Data are shown as the . . per group. Statistical significance was determined by one-way ANOVA followed by the Bonferroni posttest. Mock: saline, Ang + PKD:AngII + PKD inhibitor CID755673, and Ang + CaMKII:AngII + CaMKII inhibitor KN93.

(a)

(b)