Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis

Table 3

Information and dilution rates of antibodies.

Name	Manufacture	Item no.	Dilution rate
AKT antibody	Cell Signaling Technology	4691S	1 : 1000
p-AKT (Ser473) antibody	Cell Signaling Technology	4060S	1 : 1000
NFκB p65 antibody	Cell Signaling Technology	8242 T	1 : 1000
IκB-α antibody	Cell Signaling Technology	4814 T	1 : 800
Lamin B1 antibody	ABclonal	A11495	1 : 1500
NLRP3 antibody	ABclonal	A14233	1 : 1000
TLR4 antibody	ABclonal	A17436	1 : 1000
MyD88 antibody	ABclonal	A0786	1 : 800
PI3K p85 antibody	ABclonal	A4992	1 : 500
HIF-1α	ImmunoWay	YT2133	1 : 1000
p-PI3K (Y467/199)	ImmunoWay	YP0224	1 : 1000
β-actin antibody	Zhongshan Jinqiao	TA-09	1 : 1000
HRP-conjugated goat antimouse IgG	Zhongshan Jinqiao	ZB-5305	1 : 5000
HRP-conjugated goat antirabbit IgG	Zhongshan Jinqiao	ZB-2301	1 : 5000