Table of Contents
Conference Papers in Medicine
Volume 2013, Article ID 187835, 3 pages
http://dx.doi.org/10.1155/2013/187835
Conference Paper

Programmed Cell Death Induced by Modulated Electrohyperthermia

11st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
2Department of Veterinary Clinical Medicine, Faculty of Veterinary Science, Tottori University, Tottori 680-8553, Japan

Received 22 January 2013; Accepted 7 July 2013

Academic Editors: G. Baronzio, M. Jackson, and A. Szasz

This Conference Paper is based on a presentation given by Meggyesházi Nóra at “Conference of the International Clinical Hyperthermia Society 2012” held from 12 October 2012 to 14 October 2012 in Budapest, Hungary.

Copyright © 2013 Meggyesházi Nóra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Modulated electrohyperthermia (mEHT) is a noninvasive technique for targeted tumor treatment. Method. HT29 human colorectal carcinoma cell line xenografted to both femoral regions of BalbC/nu/nu mice was treated with a single shot OTM treatment. Histomorphologic, immunohistochemical analysis TUNEL assay, and R&D Apoptosis array were performed on tissue samples. Results. mEHT caused a selective tumor demolition. An upregulation of TRAIL-R2 and FAS was observed. Cleaved caspase-3 positive cells appear at the tumor periphery. Cytochrome c and AIF release was observed in line with massive TUNEL positivity. Conclusion. In HT29 colorectal cancer xenograft, mEHT caused massive caspase independent cell death.