Table of Contents
Conference Papers in Medicine
Volume 2013 (2013), Article ID 249563, 3 pages
http://dx.doi.org/10.1155/2013/249563
Conference Paper

Early Changes in mRNA and Protein Expression Related to Cancer Treatment by Modulated Electrohyperthermia

11st Department of Pathology and Experimental Cancer Research, Ulloi ut 26, Semmelweis University, Budapest 1085, Hungary
2Department of Veterinary Clinical Medicine, Faculty of Veterinary Science, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Tottori, Japan
3Molecular Medicine Research Group, Hungarian Academy of Sciences, Budapest, Hungary
4MTA-SE Tumor Progression Research Group, Budapest, Hungary

Received 16 January 2013; Accepted 26 May 2013

Academic Editors: G. F. Baronzio, M. Jackson, and A. Szasz

This Conference Paper is based on a presentation given by Nóra Meggyesházi at “Conference of the International Clinical Hyperthermia Society 2012” held from 12 October 2012 to 14 October 2012 in Budapest, Hungary.

Copyright © 2013 Nóra Meggyesházi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Modulated electrohyperthermia (mEHT), generated by capacitive coupled, modulated 13.56 MHz radiofrequency, is a noninvasive technique for targeted tumor treatment based on elevated ion concentration and electric admittance in malignant tumors. In this study, we tested early changes in protein expression related to tumor destruction upon a single shot of 30-minute mEHT treatment of xenografted human colorectal cell line (HT29) implanted into the femoral region of Balb/c nu/nu mice. Treatment-related mRNA expression profiling was done using the human genome U133 Plus 2.0 Arrays. Apoptosis protein arrays and immunohistochemistry were performed for validating changes at the protein level. The mEHT treatment resulted in major expression changes in 48 genes including several heat-shock proteins. Apoptosis protein arrays revealed the upregulation of death receptors, Bcl-2 superfamily mitochondrial apoptosis regulatory proteins, and heat-shock proteins, which were also confirmed in situ. Within 24-hour post-treatment, mEHT resulted in the upregulation apoptosis induction and heat-shock-related gene and protein expression in HT29 colorectal cancer xenografts contributing to tumor destruction.