Table of Contents
Conference Papers in Science
Volume 2014, Article ID 147858, 5 pages
Conference Paper

PLLA/Fibrin Tubular Scaffold: A New Way for Reliable Endothelial Cell Seeding

1STEBICEF, University of Palermo, Viale delle Scienze, Building 16, 90128 Palermo, Italy
2DICAM, University of Palermo, Viale delle Scienze, Building 8, 90128 Palermo, Italy

Received 7 December 2013; Accepted 10 March 2014; Published 12 May 2014

Academic Editors: A. Lepedda and J. C. Rodriguez-Cabello

This Conference Paper is based on a presentation given by Vincenzo La Carrubba at “LIAC Meeting on Vascular Research 2013” held from 18 September 2013 to 21 September 2013 in Alghero, Italy.

Copyright © 2014 Salvatrice Rigogliuso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In the present work a simple and quick technique for cell seeding into tubular-shaped scaffolds, which allows a homogeneous cell distribution, was tested. The poly-L-lactide (PLLA) scaffolds, prepared via diffusion induced phase separation (DIPS), were filled with fibrin gel in order to obtain a hybrid scaffold for Vascular Tissue Engineering applications. The formation of immobilized fibrin networks on the inner surface of the tubular scaffolds was observed using confocal microscopy and SEM. Morphological analysis of the so-obtained scaffold revealed that the fibrin gel is uniformly distributed on the internal surface of the scaffold, leading to an organized structure. Moreover a penetration of the gel into the porous wall of the scaffold was observed. The in vitro endothelial cell cultures carried out in the scaffolds highlighted a faster cell proliferation inside the hybrid scaffold with respect to simple PLLA scaffold. Results show that the fibrin/PLLA hybrid scaffold may be favourably used for Vascular Tissue Engineering applications.