Table of Contents
Conference Papers in Science
Volume 2014 (2014), Article ID 391242, 4 pages
Conference Paper

Conformation and Physical Structure of Tropoelastin from Human Vascular Cells: Influence of Cells Lipid Loading

1Physique des Polymères, Institut Carnot, CIRIMAT UMR 5085, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 04, France
2Centro de Investigation Cardiovascular, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain

Received 4 November 2013; Accepted 19 March 2014; Published 12 May 2014

Academic Editors: V. La Carrubba, A. Lepedda, and J. C. Rodriguez-Cabello

This Conference Paper is based on a presentation given by Valerie Samouillan at “LIAC Meeting on Vascular Research 2013” held from 18 September 2013 to 21 September 2013 in Alghero, Italy.

Copyright © 2014 Valerie Samouillan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aggregated low density lipoproteins (agLDL) contribute to massive intracellular cholesteryl ester (CE) accumulation in human vascular smooth muscle cells (VSMC). Our aim was to determine the conformational and physical structure of agLDL and elastic material produced either by control human VSMC or by agLDL-loaded human VSMC (agLDL-VSMC). At the conformational level scanned by FTIR spectroscopy, a new undefined, probably non-H-bonded, structure for tropoelastin produced by agLDL-VSMC is revealed. By differential scanning calorimetry, a decrease of water affinity and a drop of the glass transition associated with aggregated tropoelastin (from 200°C to 159°C) in the supernatant from agLDL VSMC are evidenced. This second phenomenon is due to an interaction between agLDL and tropoelastin as detected by the weak specific FTIR absorption band of agLDL in supernatant from agLDL-loaded VSMC.