Biocompatibility Issues of Next Generation Decellularized Bioprosthetic Devices
Figure 2
Alpha-gal comparative detection with GS-IB4 isolectin and M86 antibody. Alpha-gal epitopes revealed by immunofluorescence for M86 antibody (red) and GS-IB4 isolectin costaining (green) in native conditions (a). After TriCol decellularization (b), epitopes are no longer immunologically detectable by M86, while GS-IB4 reveals an unspecific attraction to matrix fibers. Note in (b) the full absence of Hoechst signal (blue), meaning an effective decellularization with total removal of nuclear material. Scale bar: 100 μm. Image previously published in Naso et al. [16].
