Differential Effect of Hypoxia in Human and Mouse Vascular Smooth Muscle Cell Migration through LRP1-pPyk2-MMP-9 Axis

Figure 2

Time-course of hypoxic modulation of HIF-1α and SREBPs levels in hVSMC and mVSMC. hVSMC and mVSMC were exposed to either normoxia or hypoxia for increasing times. ((a), (b)) Representative Western blot analysis showing HIF-1α levels. β-tubulin was used as loading control. Bar graphs represent quantification of HIF-1α levels in normoxia (N, gray bars) or hypoxia (H, black bars). Real time PCR experiments showing the effect of hypoxia on SREBP-1 ((c), (d)) and SREBP-2 ((e), (f)) mRNA levels in hVSMC ((c) and (e), resp.) and mVSMC ((d) and (f), resp.). Results from two independent experiments performed in duplicate are expressed as mean ± SEM. versus normoxic cells.

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