Research Article

Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes

Figure 3

Saturation isotherm of [3H]Ins(1,4,5)P3 binding to human platelet membranes. Each point is the mean of duplicate determination. Binding assays were carried out as described in Section 2. Nonspecific binding was determined in the presence of 10  M D-Ins(1,4,5)P3. A Scatchard plot of [3H]Ins(1,4,5)P3 binding is shown in the inset. B = [3H]Ins(1,4,5)P3 specifically-bound (fmol/mg protein), B/F = the ratio of specifically-bound to free ligand in fmol of Ins(1,4,5)P3 (fmol/mg protein nM). For this particular experiment, binding indices are = 427.56 fmol/mg protein = 21.73 nM; and the correlation coefficient ( ) = 0.98.
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