Validated Stability Indicating HPTLC Method for the Determination of Dutasteride in Pharmaceutical Dosage Forms

<table>Chromatogram obtained after oxidative degradation of dutasteride. H<sub>2</sub>O<sub>2</sub>, 30% v/v, refluxed for 3 h at 50°C, Peak 1 = Degradant (<svg height="16.637501" id="M32" style="vertical-align:-4.77652pt" version="1.1" viewbox="0 0 66.4375 16.637501" width="66.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.6375)">
<g transform="translate(72,-58.69)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑅</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.7,60.37)">
<tspan style="font-size: 8.75px; " x="0" y="0">𝑓</tspan>
</text>
<text transform="matrix(1,0,0,-1,-52.82,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">=</tspan>
<tspan style="font-size: 12.50px; " x="12.027886" y="0">0</tspan>
<tspan style="font-size: 12.50px; " x="18.279387" y="0">.</tspan>
<tspan style="font-size: 12.50px; " x="21.405136" y="0">3</tspan>
<tspan style="font-size: 12.50px; " x="27.656635" y="0">9</tspan>
</text>
</g>
</g>
</svg>), Peak 2 = Degradant (<svg height="16.825001" id="M33" style="vertical-align:-4.77652pt" version="1.1" viewbox="0 0 66.4375 16.825001" width="66.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.825)">
<g transform="translate(72,-58.54)">
<text transform="matrix(1,0,0,-1,-71.95,63.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑅</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.7,60.22)">
<tspan style="font-size: 8.75px; " x="0" y="0">𝑓</tspan>
</text>
<text transform="matrix(1,0,0,-1,-52.82,63.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">=</tspan>
<tspan style="font-size: 12.50px; " x="12.027886" y="0">0</tspan>
<tspan style="font-size: 12.50px; " x="18.279387" y="0">.</tspan>
<tspan style="font-size: 12.50px; " x="21.405136" y="0">5</tspan>
<tspan style="font-size: 12.50px; " x="27.656635" y="0">5</tspan>
</text>
</g>
</g>
</svg>), Peak 3 = dutasteride (<svg height="16.637501" id="M34" style="vertical-align:-4.77652pt" version="1.1" viewbox="0 0 66.4375 16.637501" width="66.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.6375)">
<g transform="translate(72,-58.69)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑅</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.7,60.37)">
<tspan style="font-size: 8.75px; " x="0" y="0">𝑓</tspan>
</text>
<text transform="matrix(1,0,0,-1,-52.82,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">=</tspan>
<tspan style="font-size: 12.50px; " x="12.027886" y="0">0</tspan>
<tspan style="font-size: 12.50px; " x="18.279387" y="0">.</tspan>
<tspan style="font-size: 12.50px; " x="21.405136" y="0">7</tspan>
<tspan style="font-size: 12.50px; " x="27.656635" y="0">1</tspan>
</text>
</g>
</g>
</svg>).</table>

Chromatography Research International

fig3

Figure 3

Figure 3: Validated Stability Indicating HPTLC Method for the Determination of Dutasteride in Pharmaceutical Dosage Forms 