Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms

Table 6

Robustness of the method.

Parameter		Amount of vinpocetine added (μgmL^−1)	Amount of vinpocetine detected (Mean ± SD)*	%RSD
Change in mobile phase composition	Buffer : ACN = 38 : 62	200	200.33 ± 0.29	0.14
	Buffer : ACN = 40 : 60	200	200.47 ± 0.29	0.14
	Buffer : ACN = 42 : 58	200	200.56 ± 0.55	0.27
Change in column temperature	25°C	200	200.43 ± 0.71	0.35
	30°C	200	200.36 ± 0.50	0.23
	35°C	200	199.88 ± 0.56	0.28
Change in flow rate	0.9 mLmin^−1	200	200.08 ± 0.65	0.33
	1.0 mLmin^−1	200	200.41 ± 0.31	0.15
	1.1 mLmin^−1	200	200.46 ± 0.61	0.31

*Mean ± Standard Deviation of six replicates.