Development and Validation of Selective High-Performance Liquid Chromatographic Method Using Photodiode Array Detection for Estimation of Aconitine in Polyherbal Ayurvedic Taila Preparations

Table 3

Accuracy study of the proposed method for aconitine.

Amount detected in sample (μg/mL)	Amount of standard drug added (μg/mL)	Amount of drug recovered (μg/mL)*	% Recovery*
Varnaraksasa taila
28.3	0	27.6±0.4	97.7±1.6
28.3	14	41.7±0.2	98.6±0.5
28.3	28	56.2±0.3	99.9±0.6
28.3	42	69.2±0.4	98.5±0.5
Vipritmalla taila
32.2	0	31.8±0.2	98.7±0.6
32.2	16	47.9±0.4	99.4±0.2
32.2	32	65.4±0.3	101.8±0.5
32.2	48	80.4±0.5	100.2±0.7

*Mean value ± relative standard deviation (% RSD, 𝑛=3).