Table of Contents
Chromatography Research International
Volume 2012 (2012), Article ID 596807, 8 pages
Research Article

A Comparison of Methodical Approaches to Fingerprinting of the Volatile Fraction from Winter Savory (Satureja montana)

1Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland
2Department of Pharmacognosy with Medicinal Plant Unit, Faculty of Pharmacy, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland
3Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Lublin, Collegium Pharmaceuticum, 4a Chodźki Street, 20-093 Lublin, Poland

Received 14 August 2011; Accepted 3 October 2011

Academic Editor: Yvan Vander Heyden

Copyright © 2012 Józef Rzepa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


It was the aim of this study to compare the efficiency of the different essential oil extraction methods upon the two winter savory (Satureja montana) samples of different origin. The compared techniques were the headspace gas chromatography with mass spectrometric detection (HS-GC/MS) run at the two different headspace temperatures (i.e., at 80 and 100°C) and the three different steam distillation techniques preceding the GC/MS analysis. HS-GC/MS is considered as the technique of the first choice, and the compared steam distillation techniques are recommended, respectively, by Polish Pharmacopoeia, European Pharmacopoeia, and the Polish Patent. Adequate conclusions were drawn as to the advantage of HS-GC/MS (not having the pharmacopoeial recommendation) over the different steam distillation techniques and the drawbacks of each individual analytical procedure were discussed.