A Comparison of Methodical Approaches to Fingerprinting of the Volatile Fraction from Winter Savory (Satureja montana)
Table 1
Volatile compounds in Satureja montana (lot 1 and lot 2), their respective retention times (), and semi-quantitative evaluation of their relative contributions (%) to the overall volatile fraction* depending on the applied extraction method (HS-80: headspace at 80°C; HS-100: headspace at 100°C; Deryng: mode 1; Clevenger: mode 2).
Volatile compound
α-Thujene
α-Pinene
Camphene
β-Myrcene
1-Octen-3-ol
Phelandrene
α-Terpinene
D-Limonene
p-Cymene
γ-Terpinene
β-Terpineol
β-Linalool
Borneol
p-Mentha-3,6-diene-2,5-dione
Carvacrol
Caryophyllene
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Retention time (min)
6.61
6.81
7.26
7.77
7.99
8.24
8.38
8.64
8.82
9.13
9.55
9.85
11.50
13.06
13.49
14.62
Peak Area (%)
HS-80
1.61
0.87
—
0.98
1.47
—
0.94
—
14.51
8.01
1.29
—
++
3.12
68.48
—
L1
HS-100
1.31
0.86
0.35
1.71
4.30
++
1.79
—
21.92
9.69
3.07
1.37
++
11.49
44.82
1.18
Deryng
0.65
0.35
0.18
0.62
1.57
—
0.89
—
12.51
6.03
0.52
0.53
++
—
76.16
—
Clevenger
—
+
—
—
0.39
—
—
—
17.77
1.42
—
+
++
—
79.90
—
HS-80
—
11.79
5.10
—
—
—
—
12.94
32.37
—
5.10
6.92
++
—
19.63
—
L2
HS-100
—
4.77
2.09
0.19
—
—
0.56
7.64
20.46
3.10
3.50
6.06
++
1.62
31.73
3.60
Deryng
—
+
—
—
—
—
—
9.70
48.27
1.57
—
7.89
++
++
2.73
2.88
*values estimated from the respective peak heights. + data falling between the limit of detection (LOD) and the limit of quantification (LOQ). ++ lack of an adequate (i.e., enabling quantification) peak separation. — compound not detected.